<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:b1e469dc-c8ed-42ce-9267-e7221457c51a,g:dbd062a6-93ae-413c-b982-cd6edcfece0d-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://www.nautilus.bio/role/CondensedConsolidatedBalanceSheets" xlink:type="simple" xlink:href="naut-20240630.xsd#CondensedConsolidatedBalanceSheets"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CondensedConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_4fb9582f-93c0-4649-bb42-b860bcae97c7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_e71bba06-1ee8-4d49-b969-a7fb2dc56f2d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_4fb9582f-93c0-4649-bb42-b860bcae97c7" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_e71bba06-1ee8-4d49-b969-a7fb2dc56f2d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent_2f9edd86-6754-4a9c-a061-5d23b939b264" xlink:href="naut-20240630.xsd#naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_4fb9582f-93c0-4649-bb42-b860bcae97c7" xlink:to="loc_naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent_2f9edd86-6754-4a9c-a061-5d23b939b264" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_9491c2ec-0ef6-43d1-bb44-28cb52d54fac" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_4fb9582f-93c0-4649-bb42-b860bcae97c7" xlink:to="loc_us-gaap_AccountsPayableCurrent_9491c2ec-0ef6-43d1-bb44-28cb52d54fac" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_793ad78b-e884-4c01-a0a6-9c7a88e16b75" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_d60f5e10-17e4-4d36-8ad3-f418c70eb60a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_793ad78b-e884-4c01-a0a6-9c7a88e16b75" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_d60f5e10-17e4-4d36-8ad3-f418c70eb60a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent_9dd6a468-ebd4-445c-96dd-fecf2aa25726" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_793ad78b-e884-4c01-a0a6-9c7a88e16b75" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent_9dd6a468-ebd4-445c-96dd-fecf2aa25726" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_bc4f7638-65fc-4227-8aae-353d7529c0eb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_793ad78b-e884-4c01-a0a6-9c7a88e16b75" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_bc4f7638-65fc-4227-8aae-353d7529c0eb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_2475da8e-7a1b-47d4-b4b0-ada12102cda8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_f4f5c706-826f-4f83-a1be-b2be42a040c2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_2475da8e-7a1b-47d4-b4b0-ada12102cda8" xlink:to="loc_us-gaap_PreferredStockValue_f4f5c706-826f-4f83-a1be-b2be42a040c2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_784847c3-5d05-4e9e-8f7f-e94d782d82cc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_2475da8e-7a1b-47d4-b4b0-ada12102cda8" xlink:to="loc_us-gaap_CommonStockValue_784847c3-5d05-4e9e-8f7f-e94d782d82cc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_f1126698-1a2e-42de-98c4-06e846014bd3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_2475da8e-7a1b-47d4-b4b0-ada12102cda8" xlink:to="loc_us-gaap_AdditionalPaidInCapital_f1126698-1a2e-42de-98c4-06e846014bd3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_2486d7f4-a5b7-4d25-a749-ced8429e1383" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_2475da8e-7a1b-47d4-b4b0-ada12102cda8" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_2486d7f4-a5b7-4d25-a749-ced8429e1383" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_f027de13-7fcd-45a0-9c96-7c005b7b669b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_2475da8e-7a1b-47d4-b4b0-ada12102cda8" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_f027de13-7fcd-45a0-9c96-7c005b7b669b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_470fef0b-64b5-46b7-939d-7ef2ee4e0397" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_36ad7e27-b0fa-4af5-a61f-e1e2ba73f0cd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_470fef0b-64b5-46b7-939d-7ef2ee4e0397" xlink:to="loc_us-gaap_StockholdersEquity_36ad7e27-b0fa-4af5-a61f-e1e2ba73f0cd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_8a5e6cf6-9848-4e49-bef8-e23c2be7d9a6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_470fef0b-64b5-46b7-939d-7ef2ee4e0397" xlink:to="loc_us-gaap_Liabilities_8a5e6cf6-9848-4e49-bef8-e23c2be7d9a6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_1e253c6a-ce7b-4800-80bd-600bdfdd8b3c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_470fef0b-64b5-46b7-939d-7ef2ee4e0397" xlink:to="loc_us-gaap_CommitmentsAndContingencies_1e253c6a-ce7b-4800-80bd-600bdfdd8b3c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_b0f4af60-90e2-4030-9c0f-14414c7d34e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_902bed9d-3137-4812-82ef-02ca4e06be92" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b0f4af60-90e2-4030-9c0f-14414c7d34e8" xlink:to="loc_us-gaap_AssetsCurrent_902bed9d-3137-4812-82ef-02ca4e06be92" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_dc1ba9fa-afb5-401d-ae13-25e4e9f64fdd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b0f4af60-90e2-4030-9c0f-14414c7d34e8" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_dc1ba9fa-afb5-401d-ae13-25e4e9f64fdd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_5c8d2537-25ff-4099-b5dd-2bf5f5d8c3b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b0f4af60-90e2-4030-9c0f-14414c7d34e8" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_5c8d2537-25ff-4099-b5dd-2bf5f5d8c3b1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_4413f476-0e5e-4e3e-abaf-259f0aaaa9a3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b0f4af60-90e2-4030-9c0f-14414c7d34e8" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_4413f476-0e5e-4e3e-abaf-259f0aaaa9a3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesNoncurrent_62146768-f5de-4809-8fc5-a5fe56ef1d74" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecuritiesNoncurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b0f4af60-90e2-4030-9c0f-14414c7d34e8" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesNoncurrent_62146768-f5de-4809-8fc5-a5fe56ef1d74" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_7029930d-0b03-446a-b9bd-91d8e3b27e86" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_2fe13165-30d5-49d6-8925-01a1fc8bb4e5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_7029930d-0b03-446a-b9bd-91d8e3b27e86" xlink:to="loc_us-gaap_LiabilitiesCurrent_2fe13165-30d5-49d6-8925-01a1fc8bb4e5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_632e62d4-9cf1-4ede-8a4b-8f7e0fe7b398" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_7029930d-0b03-446a-b9bd-91d8e3b27e86" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_632e62d4-9cf1-4ede-8a4b-8f7e0fe7b398" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CondensedConsolidatedStatementsofOperations" xlink:type="simple" xlink:href="naut-20240630.xsd#CondensedConsolidatedStatementsofOperations"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CondensedConsolidatedStatementsofOperations" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_1608b585-b7ea-47bf-af79-264c7f9e85a4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_3a0dcdde-0c74-4382-a0e2-84a3b76473d4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_1608b585-b7ea-47bf-af79-264c7f9e85a4" xlink:to="loc_us-gaap_OperatingExpenses_3a0dcdde-0c74-4382-a0e2-84a3b76473d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_3800fe74-c782-48d8-97dd-1d80add4a4e6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_1608b585-b7ea-47bf-af79-264c7f9e85a4" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_3800fe74-c782-48d8-97dd-1d80add4a4e6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_e1b2744a-1555-40ad-b98c-3e385a3d1306" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeExpenseNonoperatingNet_610b8b80-0375-42e5-ac06-6fd5e34d121b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestIncomeExpenseNonoperatingNet"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_e1b2744a-1555-40ad-b98c-3e385a3d1306" xlink:to="loc_us-gaap_InterestIncomeExpenseNonoperatingNet_610b8b80-0375-42e5-ac06-6fd5e34d121b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingExpense_4751e2b4-ef7d-41a0-b340-c4ecf5415881" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherNonoperatingExpense"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_e1b2744a-1555-40ad-b98c-3e385a3d1306" xlink:to="loc_us-gaap_OtherNonoperatingExpense_4751e2b4-ef7d-41a0-b340-c4ecf5415881" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_e3cb78c0-8ee8-4ca8-9039-aa4e29a1b28a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_771276da-9132-42e2-a161-32a0cbb52436" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_e3cb78c0-8ee8-4ca8-9039-aa4e29a1b28a" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_771276da-9132-42e2-a161-32a0cbb52436" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_c1cb630b-2f8d-4cfa-8339-a3bc21388a94" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_e3cb78c0-8ee8-4ca8-9039-aa4e29a1b28a" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_c1cb630b-2f8d-4cfa-8339-a3bc21388a94" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CondensedConsolidatedStatementsofComprehensiveLoss" xlink:type="simple" xlink:href="naut-20240630.xsd#CondensedConsolidatedStatementsofComprehensiveLoss"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CondensedConsolidatedStatementsofComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_1b268ff2-fd6c-42f2-bb26-418f095fa4f2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_56979ed5-b85e-4dd6-9f10-9c0e03b63d02" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_1b268ff2-fd6c-42f2-bb26-418f095fa4f2" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_56979ed5-b85e-4dd6-9f10-9c0e03b63d02" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_2ad07f53-4448-4b8b-8aa7-46dff55b5af2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_1b268ff2-fd6c-42f2-bb26-418f095fa4f2" xlink:to="loc_us-gaap_NetIncomeLoss_2ad07f53-4448-4b8b-8aa7-46dff55b5af2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_42144476-f1df-4b74-990b-95073debb55d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeAvailableforsaleSecuritiesAdjustmentNetOfTaxPortionAttributableToParent_0600539e-1e71-41be-99a2-372a72fa8e5c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeAvailableforsaleSecuritiesAdjustmentNetOfTaxPortionAttributableToParent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_42144476-f1df-4b74-990b-95073debb55d" xlink:to="loc_us-gaap_OtherComprehensiveIncomeAvailableforsaleSecuritiesAdjustmentNetOfTaxPortionAttributableToParent_0600539e-1e71-41be-99a2-372a72fa8e5c" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CondensedConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="naut-20240630.xsd#CondensedConsolidatedStatementsofCashFlows"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CondensedConsolidatedStatementsofCashFlows" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_3b0263f0-1bdf-4db7-9c21-9c882df2c1a3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockPlans_62f270b2-a6cd-4bb1-9610-49c5fa8dd74d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromStockPlans"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_3b0263f0-1bdf-4db7-9c21-9c882df2c1a3" xlink:to="loc_us-gaap_ProceedsFromStockPlans_62f270b2-a6cd-4bb1-9610-49c5fa8dd74d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_67a73ba2-911b-4bff-9771-32ddf0735fcf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_3b0263f0-1bdf-4db7-9c21-9c882df2c1a3" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_67a73ba2-911b-4bff-9771-32ddf0735fcf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_5373ffca-f387-425a-9d75-22db36ae440c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleAndMaturityOfAvailableForSaleSecurities_2fb175f8-0e42-498b-90f1-c56ce6a34b4b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromSaleAndMaturityOfAvailableForSaleSecurities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_5373ffca-f387-425a-9d75-22db36ae440c" xlink:to="loc_us-gaap_ProceedsFromSaleAndMaturityOfAvailableForSaleSecurities_2fb175f8-0e42-498b-90f1-c56ce6a34b4b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_fbf5be18-aa3f-427a-a6ee-b93fc00ae581" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_5373ffca-f387-425a-9d75-22db36ae440c" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_fbf5be18-aa3f-427a-a6ee-b93fc00ae581" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireAvailableForSaleSecuritiesDebt_42e7bf1e-ce91-40bc-852a-7d8957049aed" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquireAvailableForSaleSecuritiesDebt"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_5373ffca-f387-425a-9d75-22db36ae440c" xlink:to="loc_us-gaap_PaymentsToAcquireAvailableForSaleSecuritiesDebt_42e7bf1e-ce91-40bc-852a-7d8957049aed" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccretionAmortizationOfDiscountsAndPremiumsInvestments_fe8f8d1b-d8f8-4950-8cb4-3ef7aa496815" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccretionAmortizationOfDiscountsAndPremiumsInvestments"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_AccretionAmortizationOfDiscountsAndPremiumsInvestments_fe8f8d1b-d8f8-4950-8cb4-3ef7aa496815" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_21a3e8fa-0325-44c8-8157-56fed99b788f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_NetIncomeLoss_21a3e8fa-0325-44c8-8157-56fed99b788f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_ba588c90-f887-4c8d-9fa2-0b146b9c22e9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInOperatingLeaseLiability"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_ba588c90-f887-4c8d-9fa2-0b146b9c22e9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_a153b16d-4203-43a7-824e-96d9a65d7de9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_a153b16d-4203-43a7-824e-96d9a65d7de9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_35109e91-c919-4b29-9796-9b69c4233427" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_ShareBasedCompensation_35109e91-c919-4b29-9796-9b69c4233427" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_a5cac3ee-417b-4f76-b381-434d41f77182" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_a5cac3ee-417b-4f76-b381-434d41f77182" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Depreciation_632c9d11-4a5c-40a0-827b-73e6203eef81" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Depreciation"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_Depreciation_632c9d11-4a5c-40a0-827b-73e6203eef81" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_dc84ab49-4fb0-43ea-b2ad-ed225c0658da" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_dc84ab49-4fb0-43ea-b2ad-ed225c0658da" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_26bf94fb-187c-4fa3-b1e3-4427aa366f1e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_96089f08-45a8-4730-b0ed-9a7eab3a4c99" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_26bf94fb-187c-4fa3-b1e3-4427aa366f1e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7e40902f-057c-4e37-87f0-5a8213138aed" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_59a1906f-ca87-4f4d-9d9e-693bf38fff4f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7e40902f-057c-4e37-87f0-5a8213138aed" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_59a1906f-ca87-4f4d-9d9e-693bf38fff4f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_f706b892-186c-4a33-8a73-cc655e244fcd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7e40902f-057c-4e37-87f0-5a8213138aed" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_f706b892-186c-4a33-8a73-cc655e244fcd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_c3458310-c49a-4015-a553-af3c4e819d2b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7e40902f-057c-4e37-87f0-5a8213138aed" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_c3458310-c49a-4015-a553-af3c4e819d2b" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/FairValueMeasurementsAssetsCarriedatFairValueandMeasuredonaRecurringBasisDetails" xlink:type="simple" xlink:href="naut-20240630.xsd#FairValueMeasurementsAssetsCarriedatFairValueandMeasuredonaRecurringBasisDetails"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/FairValueMeasurementsAssetsCarriedatFairValueandMeasuredonaRecurringBasisDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_2aa4caa7-8a17-4851-9a0e-f0104d6e34b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_cbc8beaa-ecf3-4e18-bfc1-51da50cca085" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_2aa4caa7-8a17-4851-9a0e-f0104d6e34b1" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_cbc8beaa-ecf3-4e18-bfc1-51da50cca085" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_09f67bb0-6e7c-437c-a6aa-798fd44b3f6a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_2aa4caa7-8a17-4851-9a0e-f0104d6e34b1" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_09f67bb0-6e7c-437c-a6aa-798fd44b3f6a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_9c121b0e-cd90-4237-9af9-0f9b81431016" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_2aa4caa7-8a17-4851-9a0e-f0104d6e34b1" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_9c121b0e-cd90-4237-9af9-0f9b81431016" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/FairValueMeasurementsUnrealizedLossesandFairValuesofAvailableforSaleSecuritiesinanUnrealizedLossPositionDetails" xlink:type="simple" xlink:href="naut-20240630.xsd#FairValueMeasurementsUnrealizedLossesandFairValuesofAvailableforSaleSecuritiesinanUnrealizedLossPositionDetails"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/FairValueMeasurementsUnrealizedLossesandFairValuesofAvailableforSaleSecuritiesinanUnrealizedLossPositionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_22079a0d-1b5d-4ede-b12c-4fe34235f97d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_e6b3655f-adbe-41bd-86f5-e7a75ae56545" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_22079a0d-1b5d-4ede-b12c-4fe34235f97d" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_e6b3655f-adbe-41bd-86f5-e7a75ae56545" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_4e64b348-f61c-4adc-b2f0-14d4428de499" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_22079a0d-1b5d-4ede-b12c-4fe34235f97d" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_4e64b348-f61c-4adc-b2f0-14d4428de499" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_70f72599-12a8-4502-8723-9b43c9caf976" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_023526f7-59a9-4866-9ad4-2d5078bf3881" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_70f72599-12a8-4502-8723-9b43c9caf976" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_023526f7-59a9-4866-9ad4-2d5078bf3881" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_39e48a1e-fb89-43fd-96e9-0cb97bac8de6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_70f72599-12a8-4502-8723-9b43c9caf976" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_39e48a1e-fb89-43fd-96e9-0cb97bac8de6" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsPropertyandEquipmentNetDetails" xlink:type="simple" xlink:href="naut-20240630.xsd#CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsPropertyandEquipmentNetDetails"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsPropertyandEquipmentNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_8ff66fad-fb90-4627-a563-746af6a703d4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_532307d2-88fb-4a82-b5a5-ee7f656511c6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_8ff66fad-fb90-4627-a563-746af6a703d4" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_532307d2-88fb-4a82-b5a5-ee7f656511c6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_94048c1e-1e3d-4f1b-9ffb-2372e04c1c72" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_8ff66fad-fb90-4627-a563-746af6a703d4" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_94048c1e-1e3d-4f1b-9ffb-2372e04c1c72" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsAccruedExpensesandOtherLiabilitiesDetails" xlink:type="simple" xlink:href="naut-20240630.xsd#CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsAccruedExpensesandOtherLiabilitiesDetails"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsAccruedExpensesandOtherLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent_52f880b5-8ef1-47a9-ad22-00da6d5bacc1" xlink:href="naut-20240630.xsd#naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_naut_AccruedResearchAndDevelopment_db6376ff-a8a2-4705-89f2-622091a8b3c3" xlink:href="naut-20240630.xsd#naut_AccruedResearchAndDevelopment"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent_52f880b5-8ef1-47a9-ad22-00da6d5bacc1" xlink:to="loc_naut_AccruedResearchAndDevelopment_db6376ff-a8a2-4705-89f2-622091a8b3c3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_6fb3ebd2-232f-4392-a20f-cb1768c975cd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent_52f880b5-8ef1-47a9-ad22-00da6d5bacc1" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_6fb3ebd2-232f-4392-a20f-cb1768c975cd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherSundryLiabilitiesCurrent_1f978493-f97a-4b80-8f59-6212007cc455" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherSundryLiabilitiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent_52f880b5-8ef1-47a9-ad22-00da6d5bacc1" xlink:to="loc_us-gaap_OtherSundryLiabilitiesCurrent_1f978493-f97a-4b80-8f59-6212007cc455" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedProfessionalFeesCurrent_6c89f035-5e80-4f3c-9e2f-e5122fc05a8c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedProfessionalFeesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_naut_AccruedLiabilitiesAndOtherLiabilitiesCurrent_52f880b5-8ef1-47a9-ad22-00da6d5bacc1" xlink:to="loc_us-gaap_AccruedProfessionalFeesCurrent_6c89f035-5e80-4f3c-9e2f-e5122fc05a8c" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsCashCashEquivalentsandRestrictedCashDetails" xlink:type="simple" xlink:href="naut-20240630.xsd#CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsCashCashEquivalentsandRestrictedCashDetails"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CompositionofCertainCondensedConsolidatedFinancialStatementLineItemsCashCashEquivalentsandRestrictedCashDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9f1f483b-e900-402c-8d18-4da9542e8f5e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalents_7bb86e49-d968-4d6c-b538-89f5fc745ba1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalents"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9f1f483b-e900-402c-8d18-4da9542e8f5e" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalents_7bb86e49-d968-4d6c-b538-89f5fc745ba1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_930ec0b2-371e-44dd-9777-b1903b7f3f80" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9f1f483b-e900-402c-8d18-4da9542e8f5e" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_930ec0b2-371e-44dd-9777-b1903b7f3f80" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CommonStockCommonStockReservedforFutureIssuanceDetails" xlink:type="simple" xlink:href="naut-20240630.xsd#CommonStockCommonStockReservedforFutureIssuanceDetails"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CommonStockCommonStockReservedforFutureIssuanceDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_bc94ce4f-e9c9-44a0-9f98-895471c98801" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_0f4b1508-f529-434e-9e46-6057fa253949" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_bc94ce4f-e9c9-44a0-9f98-895471c98801" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_0f4b1508-f529-434e-9e46-6057fa253949" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_33ed3602-2967-4646-8552-46c2ab455534" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_bc94ce4f-e9c9-44a0-9f98-895471c98801" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_33ed3602-2967-4646-8552-46c2ab455534" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CommitmentandContingenciesFuturePrincipalContractualObligationsforOperatingLeaseCommitmentsDetails" xlink:type="simple" xlink:href="naut-20240630.xsd#CommitmentandContingenciesFuturePrincipalContractualObligationsforOperatingLeaseCommitmentsDetails"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CommitmentandContingenciesFuturePrincipalContractualObligationsforOperatingLeaseCommitmentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_6794d4ce-6578-4db5-8682-1af15d5b4f6c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_d38ad38e-197f-4fdf-8088-4da0b1d48b2b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_6794d4ce-6578-4db5-8682-1af15d5b4f6c" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_d38ad38e-197f-4fdf-8088-4da0b1d48b2b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_9de7296f-18ad-417f-afa5-a24cbe656b9f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_6794d4ce-6578-4db5-8682-1af15d5b4f6c" xlink:to="loc_us-gaap_OperatingLeaseLiability_9de7296f-18ad-417f-afa5-a24cbe656b9f" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.nautilus.bio/role/CommitmentandContingenciesFuturePrincipalContractualObligationsforOperatingLeaseCommitmentsDetails_1" xlink:type="simple" xlink:href="naut-20240630.xsd#CommitmentandContingenciesFuturePrincipalContractualObligationsforOperatingLeaseCommitmentsDetails_1"/>
  <link:calculationLink xlink:role="http://www.nautilus.bio/role/CommitmentandContingenciesFuturePrincipalContractualObligationsforOperatingLeaseCommitmentsDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a87b3920-8d62-4d42-a1b9-5924299b8683" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_2ef1d062-1093-456a-8249-80fd2558e3be" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a87b3920-8d62-4d42-a1b9-5924299b8683" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_2ef1d062-1093-456a-8249-80fd2558e3be" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_56fb53f7-1583-4fb4-996b-76e6a44adc0d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a87b3920-8d62-4d42-a1b9-5924299b8683" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_56fb53f7-1583-4fb4-996b-76e6a44adc0d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_730e2928-ad2e-4ed9-b858-b39a62dd8b92" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a87b3920-8d62-4d42-a1b9-5924299b8683" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_730e2928-ad2e-4ed9-b858-b39a62dd8b92" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_6b22868c-dce2-40d1-ae85-cf9313298c4e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a87b3920-8d62-4d42-a1b9-5924299b8683" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_6b22868c-dce2-40d1-ae85-cf9313298c4e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_naut_LesseeOperatingLeaseLiabilityToBePaidDueAfterYearFour_6884d208-92cd-4f21-bc64-70a92b2b812b" xlink:href="naut-20240630.xsd#naut_LesseeOperatingLeaseLiabilityToBePaidDueAfterYearFour"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a87b3920-8d62-4d42-a1b9-5924299b8683" xlink:to="loc_naut_LesseeOperatingLeaseLiabilityToBePaidDueAfterYearFour_6884d208-92cd-4f21-bc64-70a92b2b812b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_930407c5-34a1-4c9d-a8b4-ab4fbf9fc74d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a87b3920-8d62-4d42-a1b9-5924299b8683" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_930407c5-34a1-4c9d-a8b4-ab4fbf9fc74d" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
